内皮素3在不同毛色绵羊皮肤的表达与定位

doi:10.11843/j.issn.0366-6964.2015.12.026

Abstract

Abstract:

To explore the expression of endothelin 3 in different coat color of sheep skin and the effect of EDN3 on the sheep coat color，the experiment analyzed the mRNA and protein expression level of EDN3 in sheep skin of different coat colors in different coat color ship by real-time quantitative PCR，ELISA and immunohistochemistry.1）qRT-PCR showed that the relative expressive of EDN3 mRNA in white sheep skin was 5.540 6±0.030 7，while in black was 1.005 8±0.062 0.The gene expression quantity of EDN3 in white and black skin tissue of the same individuality was 4.341 9±0.087 7 and 1.150 1±0.005 3.2）ELISA results showed that the expression of EDN3 protein in white and black were 128.424 7±2.223 4 and 25.114 4±3.248 3.The protein expression of EDN3 in white and black skin of the same individuality were 93.945 3±7.562 2 and 28.606 0±9.295 9.3）The EDN3 was located in the matrix，outer root sheath and dermal papilla of hair follicular by immunohischemistry.Experiments showed that the EDN3 expressed normally in black and white sheep skin，and the expression quantity had significant differences.The research indicated that EDN3 was indispensable to maintain the presence of the melanocyte，and didn’t affect the formation of friesian sheep.

CLC Number:

S826.2

LI Ya-nan，ZHAO Bing-ling，MA Shu-hui，HE Xiao-yan，FAN Rui-wen，WANG Hai-dong，GENG Jian-jun，DONG Chang-sheng. EDN3 Expression and Localization in Sheep Skin with Different Coat Color[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(12): 2322-2328.

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EDN3 Expression and Localization in Sheep Skin with Different Coat Color

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